Study Molecular Structure and NMR-based Simulation of the Glycoglycerolipid from Meiothermus taiwanensis NTU-220
Date Issued
2010
Date
2010
Author(s)
Yang, Kai-Jay
Abstract
This thesis was aimed to study the solution structure of glycoglycerolipid from Meiothermus taiwanensis NTU-220. The primary sequence of this glycoglycerolipid was studied and reported in this group as following: α1,6-Galp-β1,6-Galp- β1,2-GalpNAc-α1,1-Glcp-glycerol. According to previously reported, the amount of glycolipid distributed on the cell membrane is increasing with the raising temperature. This result indicated that those glycolipids might play as chaperon toward the maintenance of cell membrane integrity, and this field is still fully understudied. Therefore, we considered that study the 3D structure of oligo-glycolipid could provide the initial understanding of possible biological property and function. Combined the distance restraints from NOESY spectra and the exocyclic φ andψ torsional restraints from heteronuclear 1H-13C 3J-HMBC experiment with NMR solvent DMSO-d6, the molecular simulation provided two possible structures. We also attempt to predict the possible O-H…O hydrogen bonds by temperature coefficient and long-range COSY experiments, however, those hydrogen bonds could not directly be explained and fitted into the simulated structures, reasonablely.
Subjects
Glycolipid
Glycoglycerolipid
Molecular simualtion
3J H-C coupling constant
Type
thesis
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