The study pf behaviors of hepatocyte on different biomaterials and the application of macroelectrophoresis on tissue engineering
Date Issued
2011
Date
2011
Author(s)
Lu, Jui-Nan
Abstract
The purpose of the first part was to evaluate the behaviors of long-term (more than 4 years) cryopreserved human hepatocytes on biomaterials. Human hepatocytes could be cryopreserved for more than 4 years without losing liver-specific function and PVA was proposed to serve as an appropriate and promising substrate for the use in culturing long-term cryopreserved hepatocytes by maintaining high cell attachment and of high level of liver-specific function. In the second part, cells were cultured on a multi-biomaterials substrate (C/T substrate) both composed of chitosan and TCPS (tissue culture polystyrene) for 8 days. Cells expressed the behaviors based on the substrate under the cells, and none of cell was found that migrated from one substrate to the other substrate. And in the third part, insulin was added into the CT substrate to control the cell behaviors on the chitosan. Insulin greatly promted cell adhesion and enhanced the proliferation on chitosan. The insulin induced cellular behaviors can be removed by blocking the PI-3 kinase pathway, and cell behaviors restored to the chitosan-induced behaviors. And in the final part, macroelectrophoresis was used to detected the differentiation of neural stem/progenitor cell (NSPC). We found that the surface charge of NSPC was up-regulated during the early period of differentialtion.
Subjects
hepatocyte
cryopreservation
HepG2
chitosan
macroelectrophoresis
Type
thesis
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