Micropropagation, Vitroplant Forcing, and Performance Evaluation of Allotetraploid LO and LA Lilies
Date Issued
2010
Date
2010
Author(s)
Chen, Chian-Ting
Abstract
In this research, the rapid propagation and bulbing pattern of allotetraploid LO and LA (4n=48, 8M+40A) lilies was developed in vitro. The rosette growth and phase changes of vitroplant planting were conducted under various controlled conditions. The performance in morphological and ornamental characters was evaluated, and the amphidiploids chromosome pairing and fertility were also studied.
The efficently adventitious bud multiplication 5.0±0.9/mini-scale explants of allotetraploid LO lily was achieved on MS medium supplemented with 0.1 mg L-1 NAA and 0.1mg L-1 BA. The modified leaf pattern of vitroplant bulbing was dominated in scale leaf type on MS medium supplemented with 200 mg L-1 yeast extract, 80 mg L-1 adenine sulfate and 45g L-1 sucrose, than total modified leaves have 3.6±1.4 of inner scale leaves and outer scale have only 1.6±0.9. The temporary immersion system (TIS) produced 7.0±1.2 buds/mini-scale explants, and tend to scale of allotetraploid LO given 10 min/4hr immersion time and the liquid medium containing 0.1 mg L-1 NAA and 0.1 mg L-1 BA. The maximal shoot multiplication rate of allotetraploid LA lily was5.9±0.6 buds/mini-scale explant on MS medium supplement with 0.1 mg L-1 NAA alone. Howeven, the subsequent bulbing was characterized with larger portion of outer scales and a few inner scale leaves. The scale leaf bulbing growth and development was enhanced on MS medium supplemented with 2 g L-1 activated charcoal. The rooting of allotetraploid LA lily bulblet was improved on 1/2 MS medium or added with 2.0 mg L-1 IAA.
The vitroplants of allotetraploid LO and LA lilies were growing at various phytotron temperatures. The rosette stage of allotetraploid LO lily mainly display scale leaf type bulbing. The unfolding rate of scale leaf was 0.4/week at 25/20℃ condition considered as middle high vigor. The allotetraploid LA lily was dominant in scale type bulbing, and could be enforced by precooling treatment. Subsequently, accompany with alternation of scale and scale leaf type bulbing.The green plants with 8-10 scale leaves of allotetraploid LO lily were exposed to nature winter cooling for 4 weeks obtained 20% shoot emergence, 15/13℃ precooling for 10 weeks obtained 76.92% shoot emergence. Contrast to the diploid LO lily that given 6 weeks precooling is saturated for shoot emergence. The total timing from shoot emergence to flowering was calculated 95.3±11.1 days, characterized as middle earliness. The total timing from planting to flowering was 251~273 days, showed as precocity. The graceful flowers appear symmetric, flatten and wild inner perianthus, and thick texture superior to diploid. The meiotic behavior of pollen mother cell was examined as 12.67% of 24 bivalents on prophase I, the average pairing was 21.2±3.8 bivalents. The average of meiotic product was 4.8. The result of chromosome delay disjunction and splitting division may conduct to aneuploid and extra microspore formation. The Alexander’s staining of pollen viability was 3.08%. The yearling bulb circumference of allotetraploid LO lily was 14~20.8 cm, which revealed highly potential for two harvest of cut flower and bulb production in one cycle. The harvested yearling was also forcible to bloom by 102.4±9.7 days after planting, resemble to middle earliness.
Subjects
Allotetraploid
LO lily and LA lily
Type
thesis
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