Long form collapsin response mediator protein-1 (LCRMP-1) expression is associated with clinical outcome and lymph node metastasis in non-small cell lung cancer patients
Journal
Lung Cancer
Journal Volume
67
Journal Issue
1
Pages
93-100
Date Issued
2010
Author(s)
Chao Y.-C.
Chen H.-Y.
Hung P.-F.
Lin P.-Y.
Lee Y.-C.
Yang S.-C.
Hong T.-M.
Abstract
Collapsin response mediator protein (CRMP) family proteins are cytosolic phosphoproteins involved in semaphorin 3A-mediated neuronal cell growth cone collapse and cancer invasion. We identified a novel human isoform of CRMP family proteins named long form CRMP-1 (LCRMP-1), which was different from the known invasion suppressor, CRMP-1, in its molecular weight and the N-terminal exon-1. This study was aimed to elucidate the clinical significance of LCRMP-1 in non-small cell lung cancer (NSCLC) patients. Full-length human LCRMP-1 was cloned from lung adenocarcinoma based on the Expressed Sequence Tags (EST) database. We generated LCRMP-1 specific antibody and subsequent in vitro and in vivo invasion assays showed positive correlations between LCRMP-1 expression and lung cancer cell invasiveness. We further demonstrated that high LCRMP-1 mRNA expressions were associated with poor overall and disease-free survivals (P = 0.004 and 0.006, respectively, log-rank test) in 72 NSCLC patients. The results were confirmed in an independent cohort of 54 NSCLC patients by immunohistochemistry (P = 0.032, log-rank test). The metastatic lymph nodes showed higher LCRMP-1 expressions as compared with the paired primary lung tumors (P = 0.012, McNemar's test). In conclusion, LCRMP-1 was a cancer invasion enhancer that could be a novel prognostic biomarker in NSCLC. ? 2009 Elsevier Ireland Ltd. All rights reserved.
SDGs
Other Subjects
antibody; collapsin response mediator protein; collapsin response mediator protein 1; complementary DNA; messenger RNA; unclassified drug; adult; aged; amino terminal sequence; article; cancer cell culture; cancer invasion; controlled study; exon; expressed sequence tag; female; human; human cell; human tissue; immunochemistry; immunohistochemistry; in vitro study; in vivo study; lung adenocarcinoma; lung non small cell cancer; lymph node metastasis; major clinical study; male; molecular cloning; molecular weight; overall survival; plasmid; priority journal; prognosis; protein expression; reverse transcription polymerase chain reaction; Western blotting; Aged; Aged, 80 and over; Base Sequence; Carcinoma, Non-Small-Cell Lung; Cell Line, Tumor; Cloning, Molecular; Cohort Studies; Disease-Free Survival; Exons; Expressed Sequence Tags; Female; Humans; Lung Neoplasms; Lymphatic Metastasis; Male; Middle Aged; Molecular Sequence Data; Neoplasm Invasiveness; Neoplasm Staging; Nerve Tissue Proteins; Protein Isoforms; Tumor Markers, Biological
Type
journal article