Autophagy protects neuron from Aβ-induced cytotoxicity
Journal
Autophagy
Journal Volume
5
Journal Issue
4
Pages
502-510
Date Issued
2009
Date
2009
Author(s)
Abstract
Autophagy is a degradation pathway for the turnover of dysfunctional organelles or aggregated proteins in cells. Extracellular accumulation of β-amyloid peptide has been reported to be a major cause of Alzheimer disease (AD) and large numbers of autophagic vacuoles accumulate in the brain of AD patient. However, how autophagic process is involved in Aβ-induced neurotoxicity and how Aβ peptide is transported into the neuron and metabolized is still unknown. In order to study the role of autophagic process in Aβ-induced neurotoxicity, EGFP-LC3 was overexpressed in SH-SY5Y cells (SH-SY5Y/pEGFP-LC3). It was found that treatment with Aβ25-35, Aβ1-42 or serum-starvation induced strong autophagy response in SH-SY5Y/pEGFP-LC3. Confocal double-staining image showed that exogenous application of Aβ1-42 in medium caused the colocalization of Aβ1-42 with LC3 in neuronal cells. Concomitant treatment of Aβ with a selective α7nAChR antagonist, α-bungarotoxin (α-BTX), enhanced Aβ-induced neurotoxicity in SH-SY5Y cells. On the other hand, nicotine (nAChR agonist) enhanced the autophagic process and also inhibited cell death following Aβ application. In addition, nicotine but not α-BTX increased primary hippocampal neuronal survival following Aβ treatment. Furthermore, using Atg7 siRNA to inhibit autophagosome formation in an early step or α7nAChR siRNA to knock down α7nAChR significantly enhanced Aβ-induced neurotoxicity. Confocal double-staining imaging shows that nicotine treatment in the presence of Aβ enhanced the colocalization of α7nAChR with autophagosomes. These results suggest that α7nAChR may act as a carrier to bind with eAβ and internalize into cytoplasm and further inhibit Aβ-induced neurotoxicity via autophagic degradation pathway. Our results suggest that autophagy process plays a neuroprotective role against Aβ-induced neurotoxicity. Defect in autophagic regulation or Aβ-α7nAChR transport system may impair the clearance of Aβ and enhance neuronal death. ? 2009 Landes Bioscience.
SDGs
Other Subjects
alpha bungarotoxin; amyloid beta protein[1-42]; amyloid beta protein[25-35]; enhanced green fluorescent protein; microtubule associated protein; nicotine; nicotinic receptor; small interfering RNA; animal cell; article; autophagy; cell death; cell survival; confocal microscopy; controlled study; cytoplasm; degradation; hippocampus; human; human cell; light chain; nerve cell; nerve cell necrosis; neuroprotection; neurotoxicity; nonhuman; protein localization; rat; staining; starvation; transport kinetics
Type
journal article
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