The Quick Monitoring Methods for Chilling Tolerance ofhalaenopsis spp. and Doritaenopsis spp. and Productionnd Storage of Orchid Mycorrhizal Fungal Inocula
Date Issued
2008
Date
2008
Author(s)
Lee, Wen-Nan
Abstract
Phalaenopsis spp. and Doritaenopsis spp. are important export orchids in Taiwan. Low temperature during winter is harmful on physiological and morphological growth of Phal. spp. and Dtps. spp, The orchid mycorrhizal fungi (OMF) are beneficial to the orchids. The objectives of first study were to compare three detection methods of chilling tolerance of Phal. spp., inculding PSII photochemical efficiency(Fv/Fm), electrolyte leakage and chlorophyll meter reading(SPAD), and to study the possibilities of increasing chilling tolerance of phal. spp. by using plant growth substance. The second study were to research that using submerged fermentation (SmF), freeze-drying(FD) or lyophilization and solid state fermentation (SSF) to establish OMF’s mother inocula.n the experiment of setting up the quick monitoring methods of chilling tolerance for four species of Phal. spp. and one Dtps. spp. were treated 7 ℃ cold temperature for 10 days and re-warned for 7 days. Four species of Phal. spp. included KC1111(Phalaenopsis Taisuco Snow × Dtps. White Wonder), KC1938(Phal. Taisuco Kaaladian), KC2902(Phal. Mount. Lip × Dtps.(Mt. Beauty × Happy Valentine ‘Hamakite Beauty’), KHM469 (Phal. Brother Girly) and KHM192 (Dtps. Sinica Ruby ‘SCL16 #1’). PSII photochemical efficiency (Fv/Fm), electrolyte leakage and chlorophyll meter reading (SPAD) values were used to evaluate the indexes of chilling tolerance. The result showed that Fv/Fm were reliable index to predict the chilling tolerance of orchids. In the another experiment of increase chilling tolerance of Phalaenopsis spp. plants by using plant growth substances, pre-application of H2O2 (0.025 M) could increase chilling tolerance of KC1111, however, there had no suitable plant growth substance to increase chilling tolerance of KC1938. Overall, there need further experiment to test the concentrations, treatment munber of plant growth substances.he effects of freezing method, freeze drying process, and the use of protective agents on the viability of the Rhizoctonia spp. were studied. Liquid nitrogen freezing caused the highest level of damage to the cells with viability < 10%. Different concentrations of exogenous substances including sugars, polymers and nitrogen compounds were tested either alone or in combination with skim milk. Using 5%(w/v) skim milk combined with 5% maltodextrin as cryoprotectants to lyophilize, the survival rates of rehydrated OMF R01 and R02 pellets were 40% and 50%. Skim milk combined with maltodextrin and sucrose or fructose or xylose at 5% were the best protective agents tested alone but the viability of freeze-dried R15 pellets was always <35%. Survival of freeze-dried R19 pellets was maintained from 10% to 32% by using appropriate protective media containing combinations of skim milk, sugars and other protectants such as 5% glycine or 5% tryptone or 5% nutrient broth or 5% soytone or 5% yeast extract. However, the best cryoprotectant was 10% skim milk combined with 10% maltodextrin and 10% sucrose.The highest survival rate of rehydrated OMF R04 pellets was 40% by using 5% skim milk combined with 5% maltodextrin and 5% sucrose and 5% glycine as cryoprotectants to lyophilize, and use 5% soytone to replace the glycine, may obtain the similar result.sing solid state fermentation(SSF) to establish OMF’s mother inocula and inocula. Compare with 25 ℃ results, 4 ℃ was the suggestion storage temperature and the recommendation storage inocula to be as follows: The broomcorn millet and oat inocula of OMF R01 can store for sixteen months with no contamination. The broomcorn millet, millet and oat inocula of OMF R02 can store for sixteen months with no contamination. The millet, oat, vermiculite and perlite inocula of OMF R15 can store for six months with no contamination. The vermiculite and perlite inocula of OMF R04 and R19 can store at 4 ℃ for six months with no contamination.
Subjects
phalaenopsis
Rhizoctonia spp.
orchid mycorrhizal fungi(OMF)
submerged fermentation
freeze drying or lyophilization
solid state fermentation
Type
thesis
File(s)![Thumbnail Image]()
Loading...
Name
ntu-97-R95628141-1.pdf
Size
23.53 KB
Format
Adobe PDF
Checksum
(MD5):d071039926bb7ea243867e667558192d