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Studies on the Lowly Pathogenic Avian Influenza Virus Infections in Selected Avian Species
Date Issued
2005
Date
2005
Author(s)
Fang, Tsung-Hsiu
DOI
zh-TW
Abstract
Susceptibility to infection with avian influenza virus (AIV) was studied in healthy and immunodepressed pigeons, which were treated with the immunosuppressant cyclophosphamide (CY) before infection. Two subtypes of lowly pathogenic (LP) AIV (A/chicken/Taiwan/1209/03 H5N2 and A/chicken/Taiwan/3152/03 H6N1) were inoculated via oculonasal route. Nested reverse transcriptase-polymerase chain reaction (nested RT-PCR) and virus isolation were used as detective methods. The CY-treated and -untreated pigeons in both experiments did not shed viruses or become antibody positive throughout the 21-day observation period. All pigeons were negative for AIV RNA when trachea, lung, pancreas, spleen, kidney and rectum tissues were tested. The negative results were also obtained in noninoculated chickens which placed as contact controls in H6N1 AIV experiments. Therefore, it was concluded that the pigeons were resistant to infection with these LPAIV, and did not serve as a transmissible host, even in immune dysfunction. Following oculonasal inoculation with LPAIV (H5N2) in broiler chickens, virus was detected in trachea and rectum on 5 and 7 days postinoculation (DPI) by nested RT-PCR. AIV was also detected in myocardium and gizzard on 5 DPI, but rarely detected in brain and marrow on 5 or 7 DPI. No AI virus was prsent in breast, wing and thigh meat. The AI survey for 994 ducks, 71 pigeons and 80 pet birds in Taiwan was conducted from October 2004 to April 2005, we isolated two H1N1 AIVS from ducks. The isolation rates were 0.19% (2/1049), 0% (0/78) and 0% (0/87) in ducks, pigeons and pet birds, respectively. The partial NP genes of this two isolates were found to be closer to H1N1 avian viruses and to be different from H1N1 swine and human viruses.
Subjects
家禽流行性感冒病毒
低病原性
鴿
環磷醯胺
免疫抑制
巢式反轉錄聚合酶
連鎖反應
avian influenza virus
lowly pathogenic
pigeon
cyclophosphamide
immunosuppressive
Nested reverse transcriptase-polymerase chain reaction
SDGs
Type
thesis
File(s)
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Name
ntu-94-R92629035-1.pdf
Size
23.31 KB
Format
Adobe PDF
Checksum
(MD5):21ccf7f4712e3aeba5cdedff3efd5573