Coffee with Different Roasting Degree Could Have Neuroprotective Effect and Improve Neuroplasticity Against The Cell Damage Induced by Corticosterone
Date Issued
2014
Date
2014
Author(s)
Liu, Yi-Ting
Abstract
Depression is a prevailing psychiatric disease, which would cause socio-economic burden. WHO have predicted that unipolar depressive disorder will be the ranking 1st of the burden and disability disease by the year 2030. However, current therapies for depression cause many side effects. Hence it is imperative to look for an alternative preventive remedy. In my study, I aim to evaluate the neuroprotective effects of coffee extract at different roasting degrees on cell viability of corticosterone-induced differentiated and undifferentiated PC12 cells. Also I analyzed the contents of active compounds of coffee extract at different roasting degrees and investigated the relationship between antioxidant activity and neuroprotective effects. To further investigate the effects of coffee on neuroplasticity, I analyzed the average neurite length of damaged differentiated PC12 cells treated with coffee extracts. With increase in roasting degree, the antioxidant activity of coffee extract would rise slightly then decrease in ABTS assay. The contents of chlorogenic acid, ferulic acid, quercetin and rutin would decrease；caffeine and caffeic acid would rise then decrease with increase in roasting degree. The second crack coffee could increase cell viability for both differentiated and undifferentiated PC12 damaged by corticosterone. The neuroprotective effect of coffee extract may partially be related to the activity of lipophilic antioxidant such as chlorogenic acid lactones, but the underlying mechanism is still be investigated. Furthermore, coffee at all roasting degree could inhibit the neurite shortening of differentiated PC12 damaged by corticosterone, indicating the coffee at all roasting degree have the potential to improve neuroplasticity. In conclusion, coffee may have the possibility to prevent or delay the incidence of depression by its neuroprotective effect and improving neuroplasticity.
Subjects
憂鬱症
咖啡
PC12細胞
神經可塑性
Type
thesis
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