肌肉缺血再灌流損傷分子機轉（2/2）
Date Issued
2002-12-31
Date
2002-12-31
Author(s)
簡雄飛
DOI
902314B002411
Abstract
We studied the ischemia reperfusion injury of skeletal
muscleby oxygen free radicals (OFRs) detection with
MCLA chemiluminescence, neutrophil staining and
TUNEL. The OFRs as well as neutrophils and muscle
cell apoptosis peaked by day 2 after release of
tourniquet. These findings are interesting because the
neutrophil number and the muscle cells showing
apoptosis are also peaking on day 2. Neutrophils are
likely the major source of free radicals. The real-time
oxygen free radical monitoring in the reperfused
skeletal muscle was shown peaked on the 2nd day. This
piece of information is important since the regimen
decreasing the OFRs must be prolonged after 2nd day.
Otherwise the clinical result must be poor. The source
of this OFR was under investigated by neutrophil
staining and real-time PCR of CINC(the chemokine
for neutrophils of rats), which were shown to be
correlated. Besides, the blocking antibodies to CINC
could effectively decrease neutrophil infiltration and
OFRs. However the data was only preliminary.
Subjects
muscle
oxygen free radicals
ischemia
reperfusion injury
reperfusion injury
Publisher
臺北市：國立臺灣大學醫學院外科
Type
report
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