Profiling novel urinary markers for prostate cancer by 2D HPLC-ESI-MS
Date Issued
2010
Date
2010
Author(s)
Kuo, Yung-Yu
Abstract
Prostate cancer ranks the seventh cause of cancer-related death in Taiwan and the second in United States. The incidence and mortality rates of prostate cancer have been rising rapidly in the past decades. Currently, there are no accurate and specific biomarkers which could discriminate clinically relevant from clinically benign disease. The better indicators and progression are needed to avoid unnecessary treatment. Clinically, prostate-specific antigen (PSA) screening still seems the only way to diagnose the carcinoma of prostate, but lately researches showed that PSA is not prostate cancer specific. Finding novel biomarkers that are sensitive, specific and can be examined by non-invasive means are imperative.
In this thesis, the on-line 2D LC-MS and off-line 2D LC-MS methods are the main approaches to unravel the protein profiling information from urine samples. In order to acquire the moderate experimental results and establish the complete urine sample analysis for identifying prostate cancer, we use these two approaches and tested the effects of modifying several parameters (oven temperature, mobile phase gradient, salt concentration and so on). In online cases, though it can be automatically operated, the information obtained turned out to be unsatisfactory. In offline cases, it demanded much manpower for sample preparation and operating the LC-MS system. The results in offline cases not only show higher intensity and sensitivity than online cases, but provide more useful information.
Subjects
prostate cancer
high performance liquid chromatography (HPLC)
prostate-specific antigen (PSA)
bio-marker
electron spray ionization mass spectrometer (ESI-MS)
SDGs
Type
thesis
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