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Functional Analysis of a Chloroplast Copper/Zinc Superoxide Dismutase of Oncidium Gower Ramsey under Heat Stress
Date Issued
2006
Date
2006
Author(s)
Wu, Yi-Shan
DOI
zh-TW
Abstract
Oncidium Gower Ramsey is one of major cut flower species in Taiwan, and the flower production is all over the year. However, high temperature of summer and autumn results in the poor quality of cut flowers, by that not only the branching and floral number of inflorescence drop severely, but the length of inflorescence is shorter than that developed in cool seasons. In order to understand high temperature-related genes in inflorescence of Oncidium, DDRT-PCR technique was used. 298 gene fragments shown differential expression were cloned and sequenced. After Blast alignment, six clones possessing high homology with known genes related to oxidative stress were selected. Among several oxidative stress related genes, three complete cDNA sequences coding for Oncidium lipase(Olipase)、cysteine synthase(OOASTL) and chloroplast CuZnSOD(OSOD) were cloned by 5’RACE. For high temperature stress, AtHSP101 promoter were used to drive sense and antisense OSOD in Arabidopsis thaliana. Even though OSOD and CSD2 (chloroplast CuZnSOD of Arabidopsis) were induced by high temperature, photooxidative stress still occurred in Oncidium and Arabidopsis. csd2 mutant unexpectedly had higher expression of CSD2 than wild type, and had more branch number in both moderate and high growth temperature(35℃). The phenotype of Arabidopsis is not directly affected by OSOD or CSD2, but the enhancement of the active oxygen-scavenging system that leads to increased oxidative stress protection and delayed the harm caused by high temperature in transgenic plants.
Subjects
氧化逆境
活性氧
超氧歧化酶
ROS
SOD
heat stress
Type
other