Analysis of Adenosine Triphosphate and Glutathione through Gold Nanoparticles Assisted Laser Desorption/Ionization Mass Spectrometry
Resource
Analytical Chemistry 79 (13): 4852-4859
Journal
Analytical Chemistry
Journal Volume
79
Journal Issue
13
Pages
4852-4859
Date Issued
2007
Date
2007
Author(s)
Huang, Yu-Fen
Abstract
This paper describes the use of aptamer-modified gold nanoparticles (Apt-AuNPs) as selective probes and AuNPs as the surface-assisted laser desorption/ionization (SALDI) matrixes for the determination of adenosine triphosphate (ATP) by mass spectrometry (MS). The aptamers were covalently attached to the surface of AuNPs to form Apt-AuNPs that provided selectivity toward ATP. However, Apt-AuNPs are less efficient laser desorption/ionization (LDI) matrixes when compared to AuNPs. By using Apt-AuNPs as selective probes and AuNPs as LDI matrixes, the MS approach provided the limit of detection (LOD) for ATP at a signal-to-noise ratio of 3 of 0.48 microM. When compared to conventional organic matrixes (e.g., 2,5-dihydroxybenzoic acid), AuNPs as LDI matrixes provide a number of advantages, including ease of preparation, selectivity, sensitivity, and repeatability. Sequential analysis of ATP and GSH in human cell lysates by SALDI with negative and positive MS modes, respectively, using Apt-AuNPs and AuNPs has been demonstrated. The present results demonstrate the practicality of the approach for monitoring the bioactivity of cells through determinations of the concentrations of ATP and GSH.
SDGs
Type
journal article
File(s)![Thumbnail Image]()
Loading...
Name
81.pdf
Size
329.41 KB
Format
Adobe PDF
Checksum
(MD5):920af824d20883cfb2264cc8035fedf6