Metabolomics Study of Ginger Essential Oil against Alcoholic Fatty Liver
Date Issued
2014
Date
2014
Author(s)
Liu, Chun-Ting
Abstract
Fatty liver is highly associated with hepatic cirrhosis, and liver cancer. The pathogenesis of fatty liver is mainly divided into two categories: non-alcoholic fatty liver disease (NAFLD) and alcoholic fatty liver disease (AFLD). Alcoholic and non-alcoholic fatty livers could be induced by alcohol consumption, obesity and hyperlipidemia. Ginger has been reported to exhibit antioxidant potential and hepatoprotective activity. In present study, a mouse model for AFLD was developed and validated by employing male C57BL/6 (B6) mice with alcohol containing liquid diet (Lieber-DeCarli diet) ad libitum. In the treatment groups, ginger essential oil (GEO) and citral, one of the major active compounds in GEO, were administered orally every day for 4 weeks. Metabolites in serum and urine samples were profiled by ultra high performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (UHPLC-QTOF-MS). Serum biochemical analysis, liver antioxidant enzyme activity and histopathological evaluation, of GEO treated mice exhibited hepatoprotective activity on alcohol-induced fatty liver. Several metabolites involved in key biochemical pathways were identified to be implicated by AFLD after four weeks of alcohol administration, such as D-glucurono-6,3-lactone, glycerol-3-phosphate, pyruvic acid, lithocholic acid, 2-pyrocatechuic acid, prostaglandin E1, methionine, L-gulono-1,4-lactone, lactate in serum samples, and serine, allantoin, 5''-methylthioadenosine, taurine in urine samples. The metabolomics result indicated these metabolites were related to AFLD. These metabolites can be use as biomarker candidates for the healthy management and clinical application on AFLD.
Subjects
酒精性脂肪肝
薑精油
酒精液態飼料
代謝體學
生物標記物
SDGs
Type
thesis
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