Effect of ginger essential oil on the physiological functions of hepatoma cells and normal hepatocytes
Date Issued
2004
Date
2004
Author(s)
Chen, Chia-Lin
DOI
zh-TW
Abstract
Abstract
The objectives of this study were to investigate the effects of various concentrations of ginger essential oil on cell viability, antioxidation and detoxification system in hepatoma cells, Hep G2 and Hep 3B, and primary rat hepatocytes. Ginger essential oil (GEO) was extracted from Zingiber officinale Roscoe by steam distillation. The cell viability of hepatoma cell lines Hep G2 and Hep 3B, which were treated with various concentrations of GEO, was investigated by MTT assay. In addition, the cell viability, antioxidation and detoxification systems of primary rat hepatocytes treated with various concentrations of GEO were also investigated. Results showed that the average yield of extraction of fresh ginger was 0.16%, the major components of the GEO were geranial (25.2 %) and citronellol (18.7 %) in GEO based on the GC-MS analysis. In MTT assay, when the Hep 3B cells were treated with high concentrations of GEO (100~200 μg/ml) for 24 hours, the inhibition percentages were significantly higher than that of the control (p＜0.05). The IC50 of Hep 3B was around 173 μg/ml of GEO. For Hep G2 treated with 50~200 μg/ml of GEO, inhibition percentages of cell viability were also significantly higher than that of the control (p＜0.05). The IC50 of Hep G2 was about 94.2 μg/ml of GEO. These results supgested that the inhibition effect of GEO on Hep G2 was much better than on Hep 3B. Under low concentration of GEO (50
Subjects
細胞生存力
薑
肝癌
cell viability
ginger
liver cancer
SDGs
Type
thesis
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