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利用cDNA microarray 分析急性白血病病患的基因表現
Date Issued
2002-07-31
Date
2002-07-31
Author(s)
陳耀昌
DOI
902314B002294
Abstract
Background: Acute leukemia, a common hematological malignancy, is clinically
divided into various subtypes according to morphology, immunophenotypes and
cytogenetic changes, and various genetic and molecular abnormalities with significant
prognostic implications could be found in many subtypes. However, because of the
large-scale data and time-consuming laboratory works needed to illustrate the gene
expression patterns, we still know little about the mechanisms of leukemogenesis,
disease progression and development of drug resistance in the patients with acute
leukemia.
Aims:
1. As a pilot study to evaluate the efficacy of cDNA microarray in exploring the
expression gene profiles in acute leukemia.
2. To explore the possible genes differentially expressed in the development of
leukemic drug resistance.
Methods:
1. Sample collection: Patients with inv(16) AML, t(7;11) AML and t(9;22) ALL
diagnosed in NTUH is selected eligible for study 歐.
2. RNA extraction and RT will be performed, and the cDNAs were labeled with
radioisotope 33P at the step of RT.
3. 33P-labeled cDNA probes will be hybridized with designed cDNA microarray.
PhospoImage system will be used for reading signals, and AtlasImage system is
used for data analysis.
Results and discussion: we find that many over-expressed genes are related to cell
cycle progression and cell survival enhancing such as c-myc and CDKN1A in AML
with inv(16); some others belong to the family of transcription activation factors, such
as basic-leucine zipper transcription factor MAFG and signal transducing adaptor
molecule (STAM) in AML with t(7;11). However, this is not always true since several
genes related to cell survival and cell cycles also are down-regulated during disease
progression, such as Cell cycle progression restoration protein 8 (CPR8) and
elongation factor 1 alpha (EF1 alpha) in ALL with t(9;22). Furthermore, different
genes are up- or down-regulated in disease relapse in different kinds of acute
leukemia. Different kinds of mechanism for drug resistance thus are proposed in
various types of leukemia. However, final common results are obtained.
divided into various subtypes according to morphology, immunophenotypes and
cytogenetic changes, and various genetic and molecular abnormalities with significant
prognostic implications could be found in many subtypes. However, because of the
large-scale data and time-consuming laboratory works needed to illustrate the gene
expression patterns, we still know little about the mechanisms of leukemogenesis,
disease progression and development of drug resistance in the patients with acute
leukemia.
Aims:
1. As a pilot study to evaluate the efficacy of cDNA microarray in exploring the
expression gene profiles in acute leukemia.
2. To explore the possible genes differentially expressed in the development of
leukemic drug resistance.
Methods:
1. Sample collection: Patients with inv(16) AML, t(7;11) AML and t(9;22) ALL
diagnosed in NTUH is selected eligible for study 歐.
2. RNA extraction and RT will be performed, and the cDNAs were labeled with
radioisotope 33P at the step of RT.
3. 33P-labeled cDNA probes will be hybridized with designed cDNA microarray.
PhospoImage system will be used for reading signals, and AtlasImage system is
used for data analysis.
Results and discussion: we find that many over-expressed genes are related to cell
cycle progression and cell survival enhancing such as c-myc and CDKN1A in AML
with inv(16); some others belong to the family of transcription activation factors, such
as basic-leucine zipper transcription factor MAFG and signal transducing adaptor
molecule (STAM) in AML with t(7;11). However, this is not always true since several
genes related to cell survival and cell cycles also are down-regulated during disease
progression, such as Cell cycle progression restoration protein 8 (CPR8) and
elongation factor 1 alpha (EF1 alpha) in ALL with t(9;22). Furthermore, different
genes are up- or down-regulated in disease relapse in different kinds of acute
leukemia. Different kinds of mechanism for drug resistance thus are proposed in
various types of leukemia. However, final common results are obtained.
Subjects
Acute myeloid leukemia
Acute lymphoblastic leukemia
cDNA microarray
Publisher
臺北市:國立臺灣大學醫學院檢驗醫學科
Coverage
計畫年度:90;起迄日期:2001-08-01/2002-07-31
Type
journal article
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