Enzyme-linked immunospot assay for interferon-gamma in the diagnosis of tuberculous pleurisy
Journal
Clinical Microbiology and Infection
Journal Volume
15
Journal Issue
2
Pages
173-179
Date Issued
2009
Author(s)
Abstract
Patients presenting with pleural effusion of undetermined aetiology were prospectively enrolled, and an enzyme-linked immunospot (ELISPOT) assay on pleural fluid and peripheral blood was performed. Forty patients were studied, including 19 with culture- or biopsy-confirmed (n = 15) or clinically compatible (n = 4) tuberculous pleurisy, and 21 with pleural effusions due to non-tuberculous causes. The sensitivity, specificity and positive and negative predictive values of the assay were 94.7%, 85.7%, 85.7% and 94.7%, respectively, on pleural fluid, and 77.8%, 90.5%, 87.5% and 82.6%, respectively, on blood. Antigen-specific, interferon-gamma-secreting T-cells were concentrated eight to ten times in pleural fluid as compared with blood. Among the seven patients not suitable for pleural biopsy and three patients whose biopsy results were non-diagnostic, nine had positive ELISPOT result with pleural fluid. The ELISPOT assay for interferon-gamma can accurately diagnose tuberculous pleurisy and is helpful for patients not suitable for pleural biopsy and those whose biopsy results are non-diagnostic. ? Journal compilation ? 2009 European Society of Clinical Microbiology and Infectious Diseases.
SDGs
Other Subjects
culture filtrate protein 10; early secretory antigenic target 6; gamma interferon; adult; aged; article; bacterium culture; clinical effectiveness; clinical trial; controlled clinical trial; controlled study; cytokine release; diagnostic accuracy; diagnostic value; enzyme linked immunospot assay; feasibility study; female; histopathology; human; major clinical study; male; peripheral blood mononuclear cell; pleura biopsy; pleura effusion; pleura fluid; priority journal; prospective study; sensitivity and specificity; T lymphocyte; tuberculous pleurisy
Type
journal article