Detection of Anti-eplieptic Drug Valproic acid by CMOS-based Microcantilever
Date Issued
2015
Date
2015
Author(s)
Wu, Sheng-Ghih
Abstract
This study demonstrates a standard CMOS-MEMS piezoresistive microcantilever biosensor with electric field enhancement of capture antibody immobilization to be a portable, real-time and quantitative biosensor for drug detection. For patients under the therapeutic drug monitoring that manages drug dosage of very narrow concentration in blood, this device shows a potential of fast turn-around-time, low cost and efficient management for point-of-care applications or personal diagnosis. The microcantilever biosensor based on immobilized antibody conformation change for specific recognition is able to yield minute induced surface stresses of cantilever deflection. Especially, this biosensor exhibits unique advantages on small-molecule detection over available label-free biosensors. Meanwhile, the microcantilever sensors were fabricated by tsmc 0.35 μm 2P4M CMOS-MEMS process with an additional gold layer coating on top of the sensing surface. The sensors were packaged into a micro-channel and its associated fluidic system. The ITO (Indium tin oxide) was embedded within the micro-channel system as to apply an electric field in a capture protein immobilized processs. Valproic acid has long been used as an antiepileptic medication, which is administered through therapeutic drug monitoring and has a narrow therapeutic dosage range of 50–100 μg/mL in blood or serum for drug effectiveness. The measurement of the antiepileptic drug Valproic acid used a single free-standing, electrical detection microcantilever biosensor in a thermally controlled system. This study showed the results of response electrical signal and associated surface stresses with respect to the valproic acid drug concentrations of 50 μg/mL, 75 μg/mL, 100 μg/mL. With the 30 volts of applied electric fields, the study achieved the valproic acid drug detection by 1.84 folds of signal response increase over a regular measurement. This study shows the capability of microcantilever biosensors for detection of small-molecule drug Valproic acid. With an introduction of the electric field for enhanced capture protein immobilization, the standard CMOS-MEMS piezoresistive microcantilever biosensor exhibits efficient use of small-molecule drug detection for potential personal diagnostics.
Subjects
microcantilever
piezoresistive
valproic acid
electrical field enhancement
Type
thesis
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