Effects dietary with Cordyceps militaris rice medium on growth and immune responses of orange-spotted grouper (Epinephelus coioides)
Date Issued
2014
Date
2014
Author(s)
Chang, Wei-Ning
Abstract
This research studied the dietary effect of Cordyceps militaris rice medium and 80% ethanol extract Cordyceps militaris rice medium on the growth, non-specific immune parameters and blood lipid of oranged-spotted grouper (Epinephelus coioides).
In experiment I, the Cordyceps militaris rice medium was supplemented at 0%, 0.25%, 0.5%, 0.75%, 1% and 1.25% to the basal diets. Each diet was fed to duplicate groups of fish (initial body weight: 4.46±0.54 g) for 42 days. In growth performances, the results show that the feed efficiency and percent weight gain of orange-spotted grouper fed Cordyceps militaris rice medium containing diets supplemented with 0.5% have better performance. The results of non-specific immune parameters show that the production ability of superoxide anion and lysozyme activity are higher in fish fed diets supplemented with 0.75% than other treatments. The alkaline phosphatase is higher in fish fed diets supplemented with 0.25% than other treatments. In blood lipid, the triglyceride decrease as the rice medium in diets increase. No significant differences (p>0.05) in cholesterol between experimental groups.
In experiment II, the 80% ethanol extracted Cordyceps militaris rice medium was supplemented at 0%, 0.25%, 0.5%, 0.75%, 1% and 1.25% to the basal diets. Each diet was fed to duplicate groups of fish (initial body weight: 4.52±0.58g) for 6 weeks. Percent weight gains and feed efficiency are higher in orange-spotted grouper fed diet containing 1% of 80% extracted ethanol Cordyceps militaris rice medium. In non-specific immune responses show that the production of superoxide anion (O2-) and lysozyme activity are higher in fish fed diets with supplemented with 0.5% extracted rice medium than other treatments. Alkaline phosphatase activity is higher in groupers fed with control diets. Triglyceride decreases as the extracted rice medium in diets increase. Groupers fed contained extracted rice medium diets have lower cholesterol than groupers fed control diets.
Subjects
北蟲草
培養米基
點帶石斑
成長
免疫
Type
thesis
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