Simultaneous DNA Detection with Synthesis of Gold Nanoparticles in Microreactor
Date Issued
2011
Date
2011
Author(s)
Tsai, Sheng-Hung
Abstract
The purpose of this thesis is to design a continuous two-phase microreactor, which applies silicon oil to avoid fouling of gold nanoparticles on channel walls. A new DNA detection method was also devoloped. Gold nanoparticles grow into different shape when single-strand DNA or double-strand DNA is added to growth process. Absorption spectrum was measured to detect target DNA.
There are four steps in the thesis. First, gold nanoparticles were synthesized in microreactor. Second, A coutinuous two-phase microreactor was designed to avoid gold nanoparticles fouling on channel walls. This design was analyzed by simulation and experiment. Third, gold nanoparticles were synthesized in the continuous two-phase microreactor. Last, the effects of single-strand DNA and double-strand DNA on synthesis of gold nanoparticles in centrifuge tube were determined.
Results are divided into two parts. The first part is continuous two-phase microreactor. The simulation results show that annular flow is formed when the velocity of water is 0.2 m/s and the velocity of water is 0.1 m/s. Vertically straitified flow is formed when the velocity of water increases, while droplet flow is formed when the velocity of water decreases. The experiment results of flow configuration show the similar trend; however, the experimental velocity of water is about two times larger than the simulation to reach the same flow configuration. The continuous two-phase microreactor was then applied to the synthesis of gold nanoparticles. The results show that fouling on channel walls was reduced significantly. The second part is DNA detection. Absorption spectrum of gold nanoparticles synthesized with single-strand DNA has red shift compared with those synthesized without DNA. There is further red shift when gold nanoparticles are synthesized with double-strand DNA.
Subjects
microreactor
two-phase flow
gold nanoparticle
fouling
DNA detection
Type
thesis
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