Ratiometric Fluorescence Mesoporous Silica Nanoparticles for Intracellular pH Sensing
Date Issued
2010
Date
2010
Author(s)
Chen, Hsueh-An
Abstract
Mesoporous silica Nanoparticles (MSNs) exhibits many advantages such as large pore volume, high surface area, rigid structure, great capability of surface modification and good biocompatibility. In this research we synthesis and characterization MSNs, with pH-sensing capability and their application for intracellular pH measurements in HeLa cell. The MSNs are labeled with FITC (a pH-sensitive dye) and RITC (a pH-insensitive dye) covalently attached to the particle surface, which serves as a ratiometric Fluorescence for increased accuracy of the pH measurements. The pH dynamic range of the sensing particles is between 5.5 and 7.5 which is suitable be used to imaging intracellular pH localization.
Key word: mesoporous, nanoparticles, ratiometric fluorescence, intracellular pH, post modification, co-condensation
Subjects
mesoporous
nanoparticles
ratiometric fluorescence
intracellular pH
post modification
co-condensation
Type
thesis
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