Analysis of chemical components and biological activity of polysaccharides from mycelia of Antrodia cinnamomea
Date Issued
2005
Date
2005
Author(s)
Hsu, Wen-Chi
DOI
zh-TW
Abstract
Antrodia cinnamomea (Niu-Chan-Chi) is a peculiar medicinal
fungus in Taiwan, which grows on the moribund inner cavity heart wood of the endemic species, Cinnamomum kanehirae. Because of its slow growth-rate in nature and limited host plant, the material is insufficient on medical treatment and research. Liquid culture to mass produce the fungus is the aim of this study. Five A. cinnamomea strains 35396, 35398, B71, B85 and B86 were used.The results showed that A. cinnamomea treated with the water-extract of C. camphora exhibited higher growth promotion effect on the biomass than that of control. Strain 35396 showed the
highest dry weight, being 5.96 ± 0.19mg/l medium. All contents of dehydrosulphurenic acid, 15α-acetyl-dehydrosulphurenic acid and dehydroeburicoic acid were increased with the addition of water-extract of C. camphora and the contents were approximately close to fruiting body. It also increased the contents of
dehydroeburicoic acid and adenosine, which exhibited biological activity. Polysaccharides were extracted from cultured mycelia of five A. cinnamomea strains. Characterization of polysaccharide by high-performance anion-exchange chromatography, showed varying yields and composition of neutral sugars. The polysaccharide profiles revealed that the addition of water-extract of C. camphora increased the production of the highest molecule weight part of polysaccharide and resulted in a direct effect on the cytotoxicity of Hela cell. Activating transcription factor 3 (ATF-3) is a transcriptional repressor that is rapidly induced in cells exposed to a wide range of stress stimuli. Polysaccharides extracted from A. cinnamomea can affect the ATF-3 expression in the protein level.
Subjects
牛樟芝
多醣體
Antrodia cinnamomea
polysaccharides
Type
thesis
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