Genetic variations and polymorphisms for porcine MSTN gene related to growth and carcass traits
Date Issued
2010
Date
2010
Author(s)
Tu, Po-An
Abstract
Myostatin gene (MSTN gene) is a member of the TGF-β superfamily that can inhibit muscle growth. It’s function as essential for regulating skeletal muscle mass. In cattle, mutations and polymorphisms of MSTN gene have been reported that having relations with “double-muscled” phenotype. The polymorphisms in the MSTN promoter region of 435/447 sites have been reported as candidate genetic markers for regulating myostatin expression and also for the myogenic process. The purpose of this study was to determine genetic variations and polymorphisms for the porcine MSTN promoter region of 435/447 two sites and its effects on porcine growth and carcass traits.
The results indicated that the MSTN promoter region of 435/447 sites can be detected AA, AG, and GG 3 genotypes, respectively. And they could be divided into 6 combined genotypes: MSTN 435GG/447AA, GA/AG, AA/GG, AA/AG, GA/AA, and AA/AA; and 3 haplotypes: MSTN 435A/447G (A haplotype), 435G/447A (B haplotype), and 435A/447A (C haplotype), respectively. Moreover, we reported that the results of a MSTN 435AA/447AA genotype and 435A/447A haplotype were only found in Chinese pig breeds and Duroc, whereas MSTN 435GG/447AA, GA/AG, and AA/GG 3 genotypes and 435G/447A, 435A/447G two haplotypes were discovered in Landrace and Yorkshire. The findings had demonstrated that we not only found complete linkage disequilibrium in Landrace and Yorkshire, but also discovered new partially incomplete linkage disequilibrium in Duroc.
For growth traits test, MSTN g.435GG individuals had significant higher ADG (P < 0.01), body weight at 70 d (P < 0.05), at 150 d (P < 0.01), and were significant lower in age of 110 kg (P < 0.01) than g.435AA individuals in Duroc. On the other hand, g.447AA individuals had a significant higher value for ADG (P < 0.01), body weight at 70 d (P < 0.01) and at 150 d (P < 0.01), and were significant lower value in age days of 110 kg (P < 0.01) than g.447GG individuals. For carcass traits test, the MSTN g.435GG individuals were thicker in carcass backfat at 10th rib and last lumbar (P < 0.05) than g.435AA individuals. And that g.435AA had higher values than g.435GG for anterior-end meat (P < 0.05), posterior-end meat (P < 0.01), total lean weight (P < 0.01), and lean weight percentage (P < 0.01). The MSTN g.447GG individuals had thinner carcass backfat at 10th rib (P < 0.01), 1st rib (P < 0.05), last rib (P < 0.05), and lower posterior-end fat (P < 0.05), but greater in posterior-end meat (P < 0.01), total lean weight (P < 0.01), and lean weight percentage (P < 0.01).
In conclusion, the genetic marker of MSTN 435/447 SNPs might be used in porcine breeding and selection programs in the future. It could also be used as specific genetic maker for distinguishing Chinese and western pig breeds. Selection for MSTN g.435G and g.447A allele are expected to increase ADG, body weight and decreasing age of 110 kg, whereas selection for MSTN g.435A and g.447G allele are expected to increase muscle of limb and total meat production, decreasing backfat thickness.
Subjects
Myostatin (MSTN) gene
Polymorphism
Porcine
average daily gain
feed efficiency
carcass
Type
thesis
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