Control of pigment granular migration in the retinula of oriental fruit fly (Bactrocera dorsalis) by nitric oxide
Date Issued
2010
Date
2010
Author(s)
Chen, Hung-Yuan
Abstract
Nitric oxide (NO) is an unstable gas molecular with short half-life. In organism, NO is produced by nitric oxide synthase (NOS), affects on the soluble guanylyl cyclase (sGC) of target cells, and lead to the increase of cyclic GMP level. In insect central nervous system, NO is supposed to have some physiological functions related to the olfaction and vision. However, the real function and mechanism of NO still need to be proved. In this study, NADPH-diaphorase was used to detect the expression of NOS in the compound eye of oriental fruit fly (Bactrocera dorsalis). The existence of NOS in the primary pigment cell and around the basement membrane was observed. Compare with the rhabdome, these two areas contain more pigments. To verify the relationship between NO and the pigments of insect retinula, the NO donor (GSNO) and the NO scavenger (C-PTIO) were injected into the retinula, and to observe the pseudopupil reflection caused by the migration of the pigment granules in light-adapted retinula cells. The pseudopupil reflection decreased in GSNO treatment group, and the retinula was kept in dark-adaptation with light stimulation, indicating that NO control the pigment granule migration in the retinula cells. According to these results, it is concluded that NO can be produced from the retinula of oriental fruit fly and control the light/dark-adaptation of retinula by regulating the migration of the pigment granules.
Subjects
Nitric oxide synthase (NOS)
retinula
pigment granule
pseudopupil
Bactrocera dorsalis
Type
thesis
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