Involvement of p29 in DNA damage responses and Fanconi anemia pathway
Journal
Carcinogenesis
Journal Volume
30
Journal Issue
10
Pages
1710 - 1716
Date Issued
2009
Author(s)
Abstract
Human p29 is a chromatin-associated protein and the silencing of p29 expression increases cell population in G1 phase and decreases phosphorylation levels of Chk1 and Chk2 in response to UV treatment. To further characterize the function of p29, U2OS and Fanconi anemia complementation group G (FA-G) cells with constitutive p29 expression have been established. Analyses of these cells identified increased phosphorylation levels of Chk1 and Chk2, which were accompanied by elevated amounts of chromatin-associated Mre11-Rad50-Nbs1 complex and ATR-IP. Monoubiquitination of the FA ID complex was restored in p29 stably expressing FA-G cells. Moreover, lower tumor incidence was observed in mp29 transgenic mice after UV irradiation. These results suggest the involvement of p29 in the DNA damage responses and Fanconi anemia pathway. ? The Author 2009. Published by Oxford University Press. All rights reserved.
SDGs
Other Subjects
ATM protein; ATR protein; checkpoint kinase 1; checkpoint kinase 2; Fanconi anemia protein; Mre11 protein; nibrin; nuclear protein; protein p29; Rad50 protein; unclassified drug; animal tissue; article; cancer cell culture; cancer incidence; carcinogenesis; controlled study; DNA damage; DNA repair; human; human cell; mouse; newborn; nonhuman; nucleotide sequence; priority journal; protein analysis; protein expression; protein function; protein localization; protein phosphorylation; protein protein interaction; transgenic mouse; ubiquitination; ultraviolet irradiation; Animals; Apoptosis; Fanconi Anemia; Fanconi Anemia Complementation Group D2 Protein; Hela Cells; Humans; Mice; Mice, Transgenic; Phosphorylation; Protein Kinases; Protein-Serine-Threonine Kinases; RNA, Small Interfering; Ubiquitin; Ultraviolet Rays
Type
journal article