Induction and Cultivation of Somatic Embryogenic Cell and It’s Bioreactor Operation Strategy(1/2)
Date Issued
2003-05-29
Date
2003-05-29
Author(s)
黃世佑
DOI
912214E002034
Abstract
Somatic embryogenesis has been
receiving much attention due to its
inherent tortipotency and favorable in
inducing elite plant species and mass
production of the plants. Artificial
seed production via somatic
embryogenesis is the future prospect in
breeding. The aim of this work is to
investigate the key factors for
developing somatic embryos and
elaborating the bioreactor configuration
suitable for mass production of the
embryo. Celery ( Apium graveolens L .)
was adopted as a model system. The
work began with inducing a callus from
leaves with SH medium containing 7.5
mM of 2,4-D, and subcultured with the
same medium containing 1 mM of 2,4-D
and 1 mM kinetin. The callus obtained
from the leaves (LC1) exhibited an
Average Growth Rate (AGR) of 0.39
day -1 on day 28. The AGR was the
objective function of the optimization.
While that obtained from stem (SC1) showed the AGR of 0.56 day -1 on day 28.
Embryogenic suspension culture for 8
days resulted in a doubling in settling
cell volume (SCV). As a preliminary
optimization of culture condition of
callus induction, Response Surface
Method was employed to search the
optimum condition for obtaining the
AGR and Dry wt./Fresh wt. Ratio. The
result showed that under 2% of sucrose,
300 ppm of NH4 + , and the initial pH of
6.2, the highest AGR(0.32 day -1 ) was
obtained (based on 16th day). The
other objective function, Dry wt./Fresh
wt. increases with increase of sucrose
concentration.
In coming months, the work will be
focused on the screening of
embryogenic callus as well as the
efficient regeneration rate of embryos.
The optimum conditions for inducing
somatic embryos will be searched by
employing fractional factorial design/
Response Surface Mmethod, taking
initial pH, concentrations of NH4 + and
sucrose/mannitol and viscosity as the
independent variables. The results will
throw light on the operation of
bioreactor.
Subjects
embryogenic cells
celery
regeneration
bioreactor
high density
embryogenic cell
embryogenic cell
Publisher
臺北市：國立臺灣大學化學工程學系暨研究所
Type
report
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