Use of an oligonucleotide probe to detect Vibrio parahaemolyticus in artificially contaminated oysters
Journal
Applied and Environmental Microbiology
Journal Volume
58
Journal Volume
58
Journal Issue
10
Journal Issue
10
Pages
3419-3422
Start Page
3419
End Page
3422
ISSN
00992240
Date Issued
1992
Author(s)
Abstract
A 26-mer oligonucleotide specific to Vibrio parahaemolyticus was synthesized from a 1,275-bp thermostable direct hemolysin (tdh) gene. This oligonucleotide probe specifically reacted with DNA from 89 of 95 V. parahaemolyticus isolates but not with DNA from other vibrios or other enteric and nonenteric organisms (n = 48). The probe hybridized with Southern blots of 0.5-kb HindIII-restricted chromosomal DNA fragments from all but five V. parahaemolyticus test isolates. The probe could be used to directly identify V. parahaemolyticus in artificially contaminated food without an isolation step.
Type
other