Angiostrongylus Cantonensis: Apoptosis of Inflammatory Cells Induced by Treatment with Mebendazole or/and Interleukin 12 in Mice
Resource
EXPERIMENTAL PARASITOLOGY v.115 n.3 pp.226-232
Journal
EXPERIMENTAL PARASITOLOGY
Journal Volume
v.115
Journal Issue
n.3
Pages
226-232
Date Issued
2007
Date
2007
Author(s)
CHUANG, CHIH-CHENG
FAN, CHIA-KWUNG
SU, KUA-EYRE
TSAI, YUEH-TING
DU, WEN-YUAN
Abstract
Angiostrongylus cantonensis is the major cause of human eosinophilic meningoencephalitis. ICR mice were infected orally with 35 infective larvae and sacrificed at 4-14 days, 25 days or 32 days post infection (dpi ) for pathological and immunocytochemical examinations. In the non-treated group, no apoptosis signal was found in the meninges or parenchyma of the brains (4-14 dpi). Only a few apoptotic cells were noticed at 25 dpi (3%) and 32 dpi (10%). In the groups, the animals were given a single dose of mebendazole( 20 mg/kg, per os at various times) or injections of interleukin 12 (IL- 12) (10 ng/daily, intraperitoneally), all the animals were sacrificed at 14 dpi; the number of apoptotic cells was increased (17 -21%). In the group that received a single dose of mebendazole (4dpi) in combination with IL-12 injections (4-13dpi), mild meningitis was observed, and most of the infiltrated inflammatory cells were in the apoptotic program (55%). Taken together, apoptosis of the inflammatory cells (most were eosinophils) could be induced when the infected mice were treated with mebendazole or/and IL- 12. (c) 2006 Elsevier Inc. All rights reserved .
Subjects
Angiostrongylus cantonensis
apoptosis