OSM與LIF蛋白質對HepG2與K562細胞株生長之影響
The effects of the OSM and LIF proteins on the Growth of K562 cells and the HepG2 cells
Date Issued
2004
Date
2004
Author(s)
Yang, Hui-Wen
DOI
zh-TW
Abstract
Leukemia Inhibitory factor（LIF）and Oncostatin M（OSM）are members of the interleukin-6（IL-6）cytokine family. And both of them trigger partial overlapping signals through partially shared receptor complexes in human. IL-6 cytokine family is involved in a variety of biological responses. The hematopoiesis, inflammation immune response, and oncogenesis through their effect on cell growth, survival and differentiation are included.
In current studies, the gene expression pattern of OSM, LIF and their related receptors in normal tissues and hepatoma cell line is known. OSM was expressed more abundantly in the fetal liver than in adult liver, while the expression of LIF was undetectable in normal livers. However, LIF, but not OSM, was expressed in HCC cell lines Hep3B, Huh7, and HepG2. And their receptors, LIFR, OSMR and gp130, were expressed mainly in fetal liver, Huh7 and HepG2 cell lines.
We also found that OSM and LIF could inhibit the growth of HCC and K562 cell lines. In order to enhance the effect of growth inhibition, we try to increase the OSM and LIF concentration on the local surface of the cells by recombine six kinds of recombinant proteins in the IMPACTTM-CN System. They are LIF, OSM, LIL, OIO, LIO and OIL. After prepared these proteins, we first examined their effects on the cell growth and ERK phosphorylation..
Showed that the growth of HepG2 cells about 50%, while 20% of Hep3B cell line have been suppressed by all recombinant proteins. But the biological effects of dimerizative proteins weren’t more dominant than the monomer. We also found that OSM and OIO inhibit the growth of K562 cells, while LIF, LIL, LIO and OIL do not have work. On the contrary, the growth of Huh7 was promoted by OSM, LIO, OIL and OIO. And these proteins can induce the activation of ERK in differential level.
We try to figure out what happened during the process of growth inhibition, and use the FACS analysis to examine that. As the results have showed, there was no apoptosis effect in the K562 and HepG2 under the treatment of OSM. But OSM could modulate the cell cycle and may slow down the process through each phase of cell cycle. We also make use of NDR protein which would be down-regulate during the differentiation of K562 cell. And as the result showed, NDR wouldn’t be down-regulate by OSM in our system.
Subjects
細胞生長
細胞激素
OSM
growth
K562
LIF
Type
other
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