L-Glutamine inhibits beta-aminobutyric acid-induced stress resistance and priming in Arabidopsis
Date Issued
2010
Date
2010
Author(s)
Wu, Chen-Chi
Abstract
The non-protein amino acid beta-aminobutyric acid (BABA) is not produced by plant. BABA has been known for years to be an effective inducer of resistance in various crops. BABA-pretreated Arabidopsis plants challenged with pathogen and abiotic stress will demonstrate enhanced resistance. The induced resistance is often associated with an enhanced capacity to mobilize infection-induced cellular defense responses, this process is called “priming”. Recently, BABA priming was found to be involved in resistance to osmotic and heat stress. The process of priming or hardening involves prior exposure to biotic stress or an abiotic stress making a plant more resistant to future exposure. This indicates the higher plants possess some capacity for memory, or stress imprinting.
Microarray data analyses revealed that BABA provokes an accumulation of transcripts involved in both stress signaling and Arabidopsis developmental responses. In addition, BABA was found to activate the stress-induced morphogenic response (SIMR), and also induced anthocyanin accumulation in Arabidopsis. Free amino acids analyses showed that BABA-treated Arabidopsis contain more Asparagine and Proline. Both amino acids are known to accumulate in stressed plants. This observation further indicates that BABA induces a stress imprinting response in Arabidopsis.
L-Glutamine is known to counteract the general amino acid inhibition response when supplied to the plant at high concentration. L-Glutamine treatments were found to block BABA-mediated growth inhibition (SIMR), anthocyanin accumulation, priming and bacterial protection. Alteration of the amino acid balance in BABA-treated Arabidopsis also suggests that BABA provokes a stress response by activating the general amino acid inhibition response. Our work suggests that BABA provokes a general amino acid inhibition response in Arabidopsis that leads to stress imprinting.
Subjects
Arabidopsis
β-aminobutyric acid
stress imprinting
stress-induced morphogenic response (SIMR)
Anthocyanin
Type
thesis
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