Sustained immobilization of growth factor proteins based on functionalized parylenes
Journal
ACS Applied Materials and Interfaces
Journal Volume
6
Journal Issue
24
Pages
21906-21910
Date Issued
2014
Author(s)
Chen, Y.-C.
Sun, T.-P.
Su, C.-T.
Wu, J.-T.
Lin, C.-Y.
Yu, J.
Huang, C.-W.
Chen, C.-J.
Abstract
Protein molecules immobilized on biomaterial surfaces are performed based on oriented conjugation or replaced mimicking peptides. The sustainable immobilization of growth factor proteins using functionalized parylene coatings is demonstrated in this study. Site-specific and nonspecific immobilization approaches are realized to conjugate bone morphogenetic protein (BMP-2). The binding affinities and conformational changes of BMP-2 are confirmed by QCM and SPR characterizations. Osteoinduction of stem cells is examined by ALP activity on the BMP-2 modified surfaces. Finally, immobilizations and equally sustained biological functions of vascular endothelial growth factor (VEGF) and a mimicking peptide of KLTWQELYQLKYKG (QK) are also examined and confirmed.
SDGs
Type
journal article