Studies on DNA Sequence Variation in Waxy Gene and Genetic Constituent of F2 Populations in Rice
Date Issued
2007
Date
2007
Author(s)
Jan, Ya-Shiun
DOI
zh-TW
Abstract
To study DNA sequence variation in waxy alleles and genetic recombination in early generation for the application of the marker-assisted selection (MAS), four F2 populations including TNG 67 x KS 139, TNG 67 x TCS 10, TK 9 x Koshihikari (KOSH), and TCS 10 x TKW 1, as well as their parents were used as materials. At first, DNA sequence variations of eight parental varieties were analyzed. And the true F1 individuals were identified with SSR DNA markers. The segregation of SSR DNA markers linked with waxy locus or in other chromosomes, and genetic contribution from single parent to F2 progenies were analyzed in each of four F2 populations. The copy numbers of CT repeats and one SNP (A / C) on exon 5 were identified from full length of 5000 bp waxy alleles of eight parental varieties. These variations are available for selecting individuals with various waxy genotypes. The segregation distortion observed in the SSR DNA markers closely linked with waxy locus was possibly resulted from linkage disequilibrium. However, the segregation distortion of SSR DNA markers distributed in other chromosomes was detected and varied in cross combination and chromosome. Although the genetic contribution (based on DNA markers) of parental varieties in TNG 67 x KS 139, TNG 67 x TCS 10, TK 9 x KOSH, and TCS 10 x TKW 1 were 54.4%, 56.3%, 52.1%, and 50.2%, respectively, several F2 individuals with more than 90% genetic constituents contributed from single parent were found (for example, the TNG 67 x KS 139 population). It indicated that applying MAS in F2 population to accelerate breeding program is a promising strategy. The results of this study could provide information for the improvement of rice quality and early generation selection.
Subjects
水稻
蠟質基因
澱粉
偏差分離及SSR分子標誌
rice
waxy gene
starch
segregation distortion
and SSR marker
Type
thesis
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