Development of a dietary-induced metabolic syndrome model using miniature pigs involvement of AMPK and SIRT1
Journal
European Journal of Clinical Investigation
Journal Volume
45
Journal Issue
1
Start Page
70
End Page
80
ISSN
1365-2362
Date Issued
2015-01-01
Author(s)
Liu, Chia-Hsin
Chang, Chei-Wei
Chu, Hsien-Pin
Chen, Kuen-Jaw
Mersmann, Harry J.
Chu, Chun-Han
Abstract
Background: During the progression of the metabolic syndrome (MetS), cardiovascular diseases (CVD) appear clinically in many individuals and cause death. As a result, it is essential to set up an optimal animal model to study the mechanism of MetS leading to CVD. SIRT1 and AMPK are the master regulators of lipid and carbohydrate metabolism. The objective of this study was to establish a miniature pig model of Western diet-induced MetS and investigate the role of SIRT1/AMPK during MetS development. Materials and Methods: Five-month-old Lee-Sung (LS) and Lanyu (LY) minipigs were each randomly assigned to two groups: control diet (C) and Western diet (W), in a 6-month experimental period. Results: Western diet caused obesity in both minipig models. Compared with the CLS pigs, WLS pigs exhibited hypercholesterolaemia. However, WLY pigs maintained a similar plasma lipid profile to the CLY pigs. Western diet caused a lower antioxidant capacity in the liver of both pig models. WLS pigs had higher triglyceride accumulation in the liver than CLS pigs, whereas WLY and CLY pigs had similar hepatic triglyceride accumulation. Compared with CLS pigs, WLS pigs had a lower hepatic SIRT1 expression, whereas WLY pigs had a higher expression of AMPK, FOXO1 and SIRT1 than CLY pigs. Conclusion: Long-term feeding of the Western diet to Lee-Sung miniature pigs not only caused obesity but also induced MetS and fatty liver, whereas Western diet induced obesity in Lanyu pigs without metabolic dysfunctions. SIRT1/AMPK and their downstream pathways might be one of the possible regulators for pathological obesity in Lee-Sung pigs.
Subjects
AMPK
Lanyu miniature pig
Lee-Sung miniature pig
Metabolic syndrome
Metabolically healthy obesity
SIRT1
SDGs
Type
journal article