行政院國家科學委員會專題研究計畫成果報告:明膠軟骨透明質酸三重聚合物支架進行齒芽組織工程
Date Issued
2005
Date
2005
Author(s)
DOI
932313B002105
Abstract
Tooth lost due to periodontal disease, dental caries, trauma, or varieties of genetic
disorders continuing to affect human and animals adversely at some time in their lives.
A biological regenerated tooth could replace lost tooth, the new substituted tooth
would provide a vital alter-native to currently available clinical treatments. For this
purpose, we used a tissue engineering approach to research the tooth regeneration.
Using surgical operation to remove 1.5 month-old miniature pig molar tooth before
eruption and placed into 30 mL of DMEM (Dulbecco´s modified eagle medium)+1.5
％ PC /ST (Penicillin/streptomycin) at 4°C. Dental bud tissues were washed by 1X in
PBS（Phosphate buffer saline）, minced into < 1 mm3 pieces, and then cultured in
DMEM +1.5 ％ PC /ST + 20 % FCS at 37℃ with 5 ％ CO2. After 3 weeks cell
yielded 1 x 107 cells, and seeded cells onto Gelatin-Chondroitin-Hyaluronan,
Tri-copolymer scaffold. Cultured for one week, dental stem cells were migrated into
and attached the scaffold. The cell/scaffold constructs were implanted in both
autograft into pig and xenograft into rabbit. In addition, a piece of little cell/scaffold
sample was fixed individually with 10 ％ neutral formalin and 2.5 ％
glutaraldehyde, and then growing cells were demonstrated in scaffold by histological
section analysis and Environmental Scanning Electron Microscope (ESEM)
individually. In experimental groups: 6 pigs were implanted with cells/scaffold (C/S)
into original alveolus, rabbits were implanted with C/S into subcutaneous tissue near
the end costal cartilage. After growing in pigs and rabbits for 24 and 36 weeks, There
were regenerated growing teeth could found in 3 pigs by X ray examination,
especially a recognizable 1×0.5×0.5 ㎝3 size tooth formed in swine alveolus bone by
evaluated with radiography, but the other 3 pigs were no found. In rabbit we did not
saw anything-like tooth in original implanted place. There was a tooth containing
dentin/pulp-like complex tooth structure was appeared by hematoxylin and eosin
staining in histological analysis. In control groups: removed molar tooth but did not
implanted cell/scaffold 6 pigs group and merely implanted scaffold 3 pigs group.
After growing for 24 and 36 weeks in pigs, no any regenerated tooth was grown in
original place. Our results demonstrated regeneration of tooth structures could
achieved by apply tissue engineering technique and suggested that dental stem cells
appearance in dental pulp tissues.
Subjects
dental bud cell
scaffold
tissue engineering
tooth regeneration
SDGs
Publisher
臺北市：國立臺灣大學獸醫學系暨研究所
Type
report
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