Endophytes from Taiwan yew Taxus sumatrana (Mig.) de Laub. and their antagonistic activity against plant fungal pathogens and pinewood nematode
Date Issued
2006
Date
2006
Author(s)
Hsiao, Chi-Hui
DOI
zh-TW
Abstract
Abstract
Of the 300 fungal endophytes isolated from healthy Ｔaiwan yew Taxus sumatrana, which preserved in the Applied Mycology Laboratory, Department of Plant Pathology and Microbiology, National Taiwan University, were selected and used to test their antagonistic activity towards plant causal organisms. After preliminary screening, 162 strains were chosen for dual culture with Rhizoctonia solani, a crop damping-off pathogen, to test antagonistic interactions in vitro. The results showed at least 48 strains of them were able to attack or exert adversal effects on R. solani, either by entwining, forming appressorium and penetration, and resulted in hosts malformation and growth suppression. Usually the hosts were eventually destroyed and lysed by either single or combinative detrimental effects of the endophytes. Further testing interactions of these 48 strains endophytes towards 18 plant fungal pathogens as well as pinewood nematode, Bursaphelenchus xylophilus, were carried out in dual culture in artificial media. Under light- or scanning electron-microscopy, it has been revealed again that many of the attacked etiological organisms or pinewood nematode were either deformed, lysed or their multiplication repressed. Furthermore, the entity of the endophytes were discerned by rDNA (ITS1-5.8S-ITS2) fingerprinting by blasting the genomic database in NCBI, USA. Many of the identified endophytes represented in the phyllum Ascomycota, some were comparable to those of described endophytes, however, several were newly recorded genera or species, with domestic uniqueness and deserved further study. The preliminary exploration implicated that these endophytes possessed potential being used as biocontrol agents, particularly in enhancing the vigority and preventing the decline or seedling disease in nursery.
Subjects
內生真菌
拮抗作用
松材線蟲
Taiwan yew
Taxus sumatrana
endophytic fungi
pinewood nematode
Bursaphelenchus xylophilus
antagonistic activity
Type
other
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