Protocol for detecting homologous recombination activity in cancer cells by adenovirus-based functional assay
Journal
STAR Protocols
Journal Volume
5
Journal Issue
4
ISSN
2666-1667
Date Issued
2024-12-20
Author(s)
DOI
10.1016/j.xpro.2024.103403
Abstract
Cancer cells with homologous recombination deficiency (HRD) exhibit a distinctive vulnerability to poly(ADP-ribose) polymerase inhibitors (PARPis). To address the limitations of existing methodologies incapable of providing real-time insights into homologous recombination (HR) status, we present an adenovirus-based functional assay designed to quantify cellular HR activity. Here, we delineate the step-by-step procedure for producing the adenovirus harboring an HR reporter, processing primary cells, and assessing HR activity in primary ovarian cancer cells. For complete details on the use and execution of this protocol, please refer to Lee et al.
Subjects
cancer
cell-based assays
clinical protocol
Publisher
Elsevier BV
Description
Article number 103403
Type
journal article