Biosurfactant Production by Serratia Marcescens Ss-1 and Its Isogenic Strain Sm Delta R Defective in Spnr, a Quorum-Sensing Luxr Family Protein
Resource
BIOTECHNOLOGY LETTERS v.26 n.10 pp.799~802
Journal
BIOTECHNOLOGY LETTERS
Journal Volume
v.26
Journal Issue
n.10
Pages
799-802
Date Issued
2004
Date
2004
Author(s)
WEI, YU-HONG
LAI, HSIN-CHIH
CHEN, SHAN-YU
YEH, MAO-SONG
CHANG, JO-SHU
Abstract
Serratia marcescens SS-1 and its SpnR-defective isogenic mutant, SMDeltaR , produced an extracellular surfactant able to decrease surface tension of water from 72 to 37 dyne cm(- 1) (SMDeltaR strain) and to 45 dyne cm(-1) (SS-1 strain). The biosurfactant also emulsified kerosene and diesel with a maximum emulsion index of 77% ( diesel and kerosene) for the SMDeltaR strain, and 72% ( kerosene) and 40% ( diesel) for the SS-1 strain. Deletion of spnR gene appeared to enhance biosurfactant production. Model simulations suggest that biosurfactant production by the two strains was growth- associated. The SMDeltaR strain had a yield coefficient of 22 - 32 % g dry cell(-1), which is 32 - 50% higher than that of the SS-1 strain.
Subjects
biosurfactant
emulsification
quorum sensing
Serratia marcescens
surface tension