菜鴨卵巢濾泡細胞生長及類固醇生成調控機制之研究(2/2)
Date Issued
2004
Date
2004
Author(s)
DOI
922313B002131
Abstract
The purpose of this study was to prepare the steroidogenic enzymes-- P450scc ,
3β-HSD, and P450arom, and also to establish the isolation techniques of ovarian
follicular granulosa, theca interna and theca externa cells in Tsaiya duck (Anas
platyrhynchos var. domestica). Furthermore, in order to establish steroid hormone
secretion model of ovarian follicular cells in Tsaiya duck, progesterone, testosterone
and estradiol secretion as well as expression of 3β-HSD, 17β-HSD and P450arom in
follicular cells were studied by in vitro cell culture system. Finally, the effect of
growth factors on steroids production also will be examined.
Results showed as follow:
1. The polyclonal antibodies of P450scc、3β-HSD and P450aromatase obtained
can recognize the steroid-producing tissues of cattle, pig, goat and duck by using
Western blotting.
2. The progesterone was the main steroid produced by granulosa cell, and
testosterone was the second one. The progesterone level and enzyme activities of
3β-HSD and 17β-HSD of granulosa cell increased gradually with the follicle
maturity. The estradiol secretion from granulosa cell was low, and was not
significantly different between follicles. The main steroids produce from theca
interna cell was testosterone and estradiol. The F4/5 follicles exhibited the highest
basal testosterone and estradiol secretion as well as enzyme activities of 17β-HSD
and P450arom from theca interna cell. Both of them were declined with the follicle
maturity. Besides, theca interna did not secrete progesterone. Estradiol was the main
steroid secreted by theca externa. Theca externa of F2 presented the highest estradiol
secretion and P450arom activity. Moreover, theca externa did not secrete
progesterone and testosterone.
3. The EGF and IGF-1 could stimulate the progesterone production from
granulose cells in vitro, and TGF-β did not. The FGF has no any effect in this part.
This study demonstrated the different abilities of steroid secretion among
ovarian follicular granulosa, theca interna and theca externa cells in Tsaiya duck by
individual in vitro culture system. However, the interactions between different
layers are required further study.
Subjects
Tsaiya duck
ovarian follicle
steroid hormone
Publisher
臺北市：國立臺灣大學動物科學技術學系暨研究所
Type
report
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