Inhibition of experimental autoimmune anterior uveitis by adenovirus-mediated transfer of the interleukin-10 gene
Journal
Journal of Ocular Pharmacology and Therapeutics
Journal Volume
21
Journal Issue
6
Pages
420-428
Date Issued
2005
Author(s)
Abstract
Aims: The aim of this study was to investigate the efficiency of adenoviral-mediated transfer of the interleukin (IL)-10 gene for inhibition of experimental autoimmune anterior uveitis, a rat model of human acute anterior uveitis. Uveitis was induced in the Lewis rat by simultaneous injection of melanin-associated antigen intraperitoneally (i.p.) and into the left footpad. The animals were treated by systemic administration of adenoviral construct expressing IL-10 (Ad-IL-10) or Ad-Mock carrying no cytokine transgene. Results: A significant reduction in ocular inflammation was noted for rats that received one or two divided i.p. administrations of Ad-IL-10 (one 10 × 10 9 and two 5 × 109 particles of adenoviral construct, respectively), as judged by reduced clinical scores and decreased leukocyte infiltration in the anterior chamber and confirmed by histological examinations, relative to control animals. Systemic Ad-IL-10, treatment also revealed a higher serum level of IL-10, compared to the controls. Conclusions: The results suggest that systemic adenovirus-mediated IL-10 gene therapy has an anti-inflammatory effect on immune-mediated ocular inflammation and that this approach may be promising for the treatment of acute anterior uveitis. ? Mary Ann Liebert, Inc.
SDGs
Other Subjects
interleukin 10; melanin; Adenovirus; animal experiment; animal model; anterior eye chamber; antiinflammatory activity; article; autoimmune disease; cell infiltration; controlled study; gene transfer; histology; human; human cell; iridocyclitis; leukocyte; nonhuman; rat; transgene; viral gene therapy; Adenoviridae; Animals; Anterior Chamber; Aqueous Humor; Autoimmune Diseases; Chemotaxis, Leukocyte; Disease Models, Animal; Gene Therapy; Gene Transfer Techniques; Genetic Vectors; Interleukin-10; Leukocyte Count; Lymph Nodes; Polymerase Chain Reaction; Rats; Rats, Inbred Lew; Tumor Necrosis Factor-alpha; Uveitis, Anterior
Type
journal article