Modified low-temperature extraction method for isolation of bletilla striata polysaccharide as antioxidant for the prevention of alzheimer’s disease
Journal
International Journal of Molecular Sciences
Journal Volume
22
Journal Issue
23
Date Issued
2021
Author(s)
Abstract
Amyloid-β (Aβ) peptides play a key role in Alzheimer’s disease (AD), the most common type of dementia. In this study, a polysaccharide from Bletilla striata (BSP), with strong antioxidant and anti-inflammatory properties, was extracted using a low-temperature method and tested for its efficacy against AD, in vitro using N2a and BV-2 cells, and in vivo using an AD rat model. The characterization of the extracted BSP for its molecular structure and functional groups demonstrated the effectiveness of the modified method for retaining its bioactivity. In vitro, BSP reduced by 20% reactive oxygen species (ROS) levels in N2a cells (p = 0.0082) and the expression levels of inflammation-related genes by 3-fold TNF-α (p = 0.0048), 4-fold IL-6 (p = 0.0019), and 2.5-fold IL-10 (p = 0.0212) in BV-2 cells treated with Aβ fibrils. In vivo, BSP recovered learning memory, ameliorated morphological damage in the hippocampus and cortex, and reduced the expression of the β-secretase protein in AlCl3-induced AD rats. Collectively, these findings demonstrated the efficacy of BSP for preventing and alleviating the effects of AD. ? 2021 by the authors. Licensee MDPI, Basel, Switzerland.
Subjects
Aluminum chloride
Alzheimer’s disease
Amyloid-β
Anti-inflammation
Bletilla striata
Oxidative stress
aluminum chloride
antiinflammatory agent
antioxidant
polysaccharide
polysaccharide b, Bletilla striata
reactive oxygen metabolite
Alzheimer disease
animal
cold
drug effect
female
metabolism
oxidative stress
pathology
rat
Sprague Dawley rat
Aluminum Chloride
Alzheimer Disease
Animals
Anti-Inflammatory Agents
Antioxidants
Cold Temperature
Female
Oxidative Stress
Polysaccharides
Rats
Rats, Sprague-Dawley
Reactive Oxygen Species
SDGs
Type
journal article