Improving Enzymatic Hydrolysis of Pickled mustard by Different Pretreatment
Date Issued
2011
Date
2011
Author(s)
Lu, Chia-Yi
Abstract
Because of the climate change, geopolitical and other factors, global oil resources reduced gradually. Nowadays the overall oil demand is higher than the supply, especially in emerging industrial countries, the lack of oil resources caused the high oil price, which means the era of high energy prices is coming.
After the fermentation process, pickled mustard left the large amount of residues (pickling liquor, pickled mustard waste). Because of the high salt content, it’s not easy to deal with the waste. If we do not actively deal with this problem, it will harm the surrounding environment seriously. Pickled mustard waste from Dapi township contained large amounts of salt, it probably leads to the increase of salinization of agricultural land for a long time. Therefore, my study aimed at the methods of the textural change and re-use of residue fiber to develop the ethanol production and related program. Pickled cabbage residues are treated appropriately to keep on pickled cabbage industry under environmental protection and sustainable development.
Under the pretreatment of high heat and high pressure with 2 M H2SO4, the best glucose conversion is 22%; the best xylose conversion is 45% under the high heat and high pressure combined with 0.37 M H2SO4. Compared to steam explosion pretreatment, the glucose and xylose conversion are 15% and 30%, respectively. The results showed that the conversions are not higher than the formers. In the enzymatic hydrolysis process, the pretreatment of the best glucose conversion is high pressure and high heat pretreatment with 0.37 M H2SO4, the glucose conversion is 50%, but xylose conversion is generally low. Under high heat and high pressure with 0.37M H2SO4, the pretreatment has the best conversion of glucose and xylose (total glucose conversion is 55%, total xylose conversion is 45%). It was deduced that the process of effective pretreatment can break down the lignocellulosic structure of biomass. The cleaning procedure after pre-treatment will not lead to acid/base solution left in the solid residue, and therefore the conversion efficiency of enzymatic hydrolysis is better. Using the best pretreatment condition with the hydrolysis process, the solid residue and the hydrolyzed liquid collected after treatment continue the fermentation process, the ideal total alcohol production is 218 mg/g dry weight (164 mg/g glucose and 54 mg/g xylose, respectively).
Keywords: pickled mustard, biomass ethanol, the pretreatment process, the conversion of glucose and xylose.
Subjects
pickled mustard
biomass ethanol
the pretreatment process
the conversion of glucose and xylose
Type
thesis
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