Measuring and Analyzing Epithelial Cell Elastic Backscattering Spectroscopy
Date Issued
2011
Date
2011
Author(s)
Lai, Yi-Chang
Abstract
Recently, many researches indicate that light scattering spectroscopy (LSS) is greatly influenced by the change of structure and refractive index. By analyzing the backscattering light spectroscopy of early stage cancer cells, the changes of their nuclear size and refractive index during the development of precancerous lesions can be revealed. Therefore, LSS is a promising technique for noninvasively detecting precancerous lesions in vivo.
This thesis builds up an optical system and research methods for measuring and analyzing the backscattering light of individual epithelial cells. Our optical system contains two part, one part can directly measure the LSS form living cell. The other part combines the LSS technique with low coherence interferometry (LCI) which is called Fourier domain optical coherence tomography (FD-OCT) to detect the depth resolved backscattering signal form an object. The spectroscopic optical coherence tomography (SOCT) method is adapted to find out more scattering information.
In this thesis, I use our optical system to examine the relationship between the backscattered light of cell nucleus and enlargement of cancer cell nucleus. First, I validate our optical system with polystyrene beads and then measure the backscattering spectroscopy of living cells including human embryonic palatal mesenchymal (HEPM) cells and oral squamous carcinoma cells (CA9-22). I find out the nuclear size by fitting the result with Mie theory.
Also, I characterize the axial resolution of the FD-OCT part of the system and use the system to measure the thickness of a coverslip. In the future, I will use the FD-OCT and SOCT to find out the size of polystyrene beads and then investigate the structural information of cell nucleus.
Subjects
Light scattering spectroscopy
Elastic backscattering light
Fourier domain optical coherence tomography
Spectroscopic optical coherence tomography
Epithelial cell.
SDGs
Type
thesis
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