Factors derived from human exfoliated deciduous teeth stem cells reverse neurological deficits in a zebrafish model of Parkinson's disease.
Journal
Journal of dental sciences
Series/Report No.
Journal of Dental Sciences
Journal Volume
19
Journal Issue
4
Start Page
2035-2044
ISSN
2213-8862
Date Issued
2024-10
Author(s)
Chen, Yong-Ren
Wong, Chin-Chean
Chen, Yi-No
Yang, Bing-Heng
Lee, Po-Hui
Shiau, Chia-Yang
Li, Chung-Hsing
DOI
10.1016/j.jds.2024.06.004
Abstract
Background/purpose: Mesenchymal stem cells exhibit therapeutic efficacy for brain injury. This study examined the effect of mesenchymal stem cells derived from human exfoliated deciduous teeth (SHED) on alleviating symptoms of Parkinson's disease (PD).
Materials and methods: SHED were isolated to examine the biosafety and bioavailability of stem cells derived from human exfoliated deciduous teeth-derived conditioned medium (SHED-CM) for the alleviation of PD symptoms in a 6-hydroxydopamine (6-OHDA)-induced PD zebrafish model.
Results: SHED-CM administration did not induce neurological, skin or muscle toxicity in control zebrafish at any dose, and estrogen equivalent testing showed no chronic toxicants. Induction of PD with 6-OHDA suppressed zebra SHED-CM was administered to zebrafish treated with 6-OHDA to induce PD symptoms. Similar to nomifensine, a drug with proven anti-PD potential, SHED-CM repaired the motor deficiencies in the zebrafish PD model.
Conclusion: Our results indicate the biosafety of SHED-CM and its therapeutic potential in treating PD in a zebrafish model.
Subjects
6-OHDA zebrafish model
Parkinson's disease
SHED-CM
Tangential flow filtration
Publisher
Association for Dental Sciences of the Republic of China
Type
journal article