Synthesis, structural characterization of CuInS2 quantum dots and its chemical stability in Caenorhabditis Elegans
Date Issued
2014
Date
2014
Author(s)
Wu, Dai-Ying
Abstract
Novel nano material CuInS2 quantum dots have been the major research these years. However, these kinds quantum dots’ nano toxicity were only studied in cell cytotoxicity, seldom studied about long term digestion and accumulation effects in multi-cellular organism. In previous research, they point out that quantum dots content copper element will release copper cation into organism to be harmful and toxic in long term accumulation.
In our research, we synthesis CuInS2 and ZnS shell nanoparticle by hydrothermal method. Their wavelength is located at around near infrared region (700 nm). After coating ZnS shell the wavelength will be blue shift to 620 nm, and their emission intensity will be enhance ten times.
For biological application, we use carboxylate-modified chitosan coat outside the nanoparticle uniformly by ultra-sonication. After sonication, the quantum dots’ morphology will be change to “cluster-like” circle shape, and the wavelength is red shift to 700 nm. In biological toxicity effects, we select Hela and OECM cancer cell for in vitro to test nanoparticle cytotoxicity effects in different dots. Moreover, we select Caenorhabditis elegans (C. elegans) to be the simple multi-cellular organism model to analysis the accumulation location and possible influence location inside the elegans body. To confirm the nanoparticle chemical stability, we use X-ray Absorption Near Edge Spectra (XANES) to characterize nanoparticle chemical oxidation to analysis the decomposition extent inside the elegans body. From the XANES results, it can be confirm that quantum dots did not show significant decomposition phenomenon. We can conclude that CIS and CIS/ZnS quantum dots are still stable and low cytotoxicity in C. elegans after long term digestion.
Subjects
量子點
硫化銅銦
線蟲
近邊緣吸收光譜
SDGs
Type
thesis
File(s)![Thumbnail Image]()
Loading...
Name
ntu-103-R01223165-1.pdf
Size
23.32 KB
Format
Adobe PDF
Checksum
(MD5):685ddd9cc792a5318e45616c683c698e