Bioactive peptides production by hydrolysis of porcine blood proteins in a continuous enzymatic membrane reactor
Date Issued
2009
Date
2009
Author(s)
Wei, Jen-Ting
Abstract
Porcine blood with 17~18% protein content is a good source of protein for food. In this study, hydrolysates of red blood corpuscle, blood plasma and defibrinated blood plasma were prepared by enzymatic hydrolysis with several proteases, and evaluated for the possibility of using the enzymatic processes for manufacturing bioactive peptides. It was found that the hydrolysate produced by hydrolyzing red blood corpuscle with the mixture of trypsin, chymotrypsin and thermolysin had the highest ACE inhibitory activity (IC50 = 0.58 mg/mL) and the highest scavenging effect on DPPH (scavenging effect = 65%) after 6 h and 10 h hydrolysis, respectively. For the potential protection against Alzheimer''s disease, addition of the hydrolysate into the β-amyloid(25-35) treated RBE4 cell culture could raise the survival rate of RBE4 cell from 67% to 82%. In respect of other functionalities, including reducing power, proliferation effect on the skin fibroblast WS1 cell, immunomodulatory effect, anti-proliferative activity on the tumor cell line, although the hydrolysates did not show superior capabilities than the other well-known products, this study proved that the hydrolysate did possess such functionalities mentioned above. Because of the health-promoting functions possessed by the hydrolysate of porcine red blood corpuscles, attempt was made to evaluate the possibility of manufacturing the product continuously using enzymatic membrane reactor. It was found that when the hydrolysis was carried out with an enzyme/substrate ratio of 1:5 and a residence time of 100 min, the process reached steady state in 2 h. The ACE-inhibitory activity of the product during the steady state process was 86% and its scavenging effect on DPPH was 54%. The membrane process also decolourised the enzyme-hydrolysed product, thus improving the appearance of the product. This study demonstrated that hydrolysates of porcine blood possess antihypertensive and antioxidant activities. Using red blood corpuscles as the substrate, the hydrolysis could be carried out in a membrane reactor with a mixture of proteases to produce bioactive peptides continuously. Therefore processing of porcine blood in an enzymatic membrane reactor is a potential method for producing a health-promoting product.
Subjects
porcine blood
enzymatic hydrolysis
bioactive peptides
membrane reactor
Type
thesis
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