The level of IgA antibodies to human umbilical vein endothelial cells can be enhanced by TNF-α treatment in children with Henoch-Schönlein purpura
Journal
Clinical and Experimental Immunology
Journal Volume
130
Journal Issue
2
Pages
352-357
Date Issued
2002
Author(s)
Abstract
Anti-endothelial cell antibodies (AECA) have been found to play an important role in many vascular disorders. In order to determine the presence of AECA in children with Henoch-Sch?nlein purpura (HSP), and to elucidate the pathogenic and clinical value of their measurement in this disease, AECA were detected by immunofluorescence staining and a human umbilical vein endothelial cell (HUVEC)-based enzyme-linked immunosorbent assay (ELISA) in 20 children with HSP, 10 children with juvenile rheumatoid arthritis (JRA) without vasculitis and 10 normal healthy children. Antibodies against another endothelial cells, human dermal microvascular endothelial cells (HMVEC-d) were also detected by cell-based ELISA. In some experiments, we compared the binding activity of antibodies to HUVEC with and without tumour necrosis factor-α (TNF-α) or interleukin-1 (IL-1) pretreatment. Patients with acute onset of HSP had higher serum levels of IgA antibodies, both against HUVEC and against HMVEC-d, than healthy controls (P = 0.001, P = 0.008, respectively). Forty-five per cent of patients had positive IgA AECA to HUVEC, and 35% had positive IgA AECA to HMVEC-d. The titres of IgA antibodies to HUVEC paralleled the disease activity. After TNF-α treatment, the values of IgA AECA to HUVEC in HSP patients were significantly increased (P = 0.02). For IgG and IgM AECA, there was no difference between HSP patients and controls (P = 0.51, P = 0.91). Ten JRA children without vasculitis had no detectable IgG, IgM or IgA AECA activity. The results of this study showed that children with HSP had IgA AECA, which were enhanced by TNF-α treatment. Although the role of these antibodies is not clear, IgA AECA provide another immunological clue for the understanding of HSP.
SDGs
Other Subjects
cell antibody; endothelial cell antibody; immunoglobulin G antibody; interleukin 1; tumor necrosis factor alpha; unclassified drug; anaphylactoid purpura; antibody production; article; child; clinical article; controlled study; disease activity; drug effect; enzyme linked immunosorbent assay; human; immunofluorescence test; immunoglobulin blood level; juvenile rheumatoid arthritis; priority journal; treatment outcome; vasculitis; Adolescent; Autoantibodies; Cardiolipins; Cells, Cultured; Child; Child, Preschool; Cytokines; Dermis; Endothelium, Vascular; Enzyme-Linked Immunosorbent Assay; Female; Fluorescent Antibody Technique; Humans; Immunoglobulin A; Male; Purpura, Schoenlein-Henoch; Tumor Necrosis Factor-alpha; Umbilical Veins
Type
journal article