A Study of Single cell Manipulation, Culturing, and Real-time Investigation
Date Issued
2006
Date
2006
Author(s)
Wang, Ying-Hui
DOI
zh-TW
Abstract
Cell is the smallest, biologically functional system unit in lives, and every feature in organisms is constituted by cells. The most original cells in humans are stem cells which possess the remarkable potential to be developed into various cell types in body. The mechanism remains unknown such that a study of single stem cell is becoming a highly potential approach to unveil its activities. By taking the advantages in MEMS technique of comparable size to a cell, the manufacturing, manipulation and investigation for a single cell have been made in this work.
The objective of the study is to develop a microsystem of single cell for manipulation, culturing, and real-time investigation. In manipulation for a single cell, a micro-pipette and micro-well device has been micromanufactured in order for isolation and transportation of a single cell. In cell culturing, a single cell is trapped and cultivated within a micro-well chip inside the micro-incubation culture system. The chip provides an isolated environment for a single cell in which the device is allowed to be injected with chemicals and biomarkers to stimulate the single cell. Moreover, in cell observation, the real-time investigation has been made simultaneously by both the total internal reflection fluorescence microscopy and CytovivaTM150. The images can be obtained and analyzed with the image processing software such that a great deal of bio-information for a single cell can be achieved. With such a system, the single cell morphology and protein-protein interaction can then be potentially realized. The well-integarted cell microsystem has been made for potential study for a single stem cell or its associated interaction, differentiation and communication to specific cells.
Subjects
單一細胞
即時觀測
Single cell
real-time investigation
Type
thesis
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