Molecular Cloning of a Cold-Shock Domain Protein, Zfy1, in Zebrafish Embryo
Resource
BIOCHIMICA ET BIOPHYSICA ACTA v.1433 n.1-2 pp.343-349
Journal
BIOCHIMICA ET BIOPHYSICA ACTA
Journal Volume
v.1433
Journal Issue
n.1-2
Pages
343-349
Date Issued
1999
Date
1999
Author(s)
CHANG, BEI-EN
Abstract
Cold-shock domain proteins in vertebrates contain a highly conserved domain which is related to the Escherichia coli cold-shock proteins. Here we report the cloning of a cold- shock domain protein from zebrafish embryo. Using the combination of PCR techniques with degenerate primers, 5 P RACE and 3 P RACE, the full length cDNA of a cold-shock domain protein in the zebrafish embryo was successfully cloned without constructing and screening a library. Determined from the deduced amino acid sequence, this protein is most similar to Xenopus, FRGY 1, and this newly cloned zebrafish gene was therefore designated as zfY1. szlig; 1999 Elsevier Science B.V. All rights reserved.
Subjects
Cold-shock domain
Y box
Zebrafish
Rapid amplification of cDNA ends