Studies on Silencing of 1-Aminocyclopropane-1-Carboxylate Oxidase Genes in Transgenic Banana
Date Issued
2008
Date
2008
Author(s)
Liao, Yu-Chen
Abstract
Banana (Musa spp.) is a climacteric fruits. Mh-ACO1 and Mh-ACO2 genes isolated from banana (Musa spp., AAA group) encode fruit ripening related ACC oxidase (ACO), the last enzyme in the ethylene biosynthetic pathway. Agrobacterium-mediated method and RNA interference (RNAi) were used as a strategy of Mh-ACO1 and Mh-ACO2 gene inhibition to clarify their function in banana. In the Agrobacterium-mediated method, banana (Musa ‘Pei Chiao’, AAA group) suspension cells were co-cultivated with Agrobacterium and selected with 25 mg/L G418. In 5 months, the embryos developed roots. Southern blot analysis indicated that the Mh-ACO2 silenced transgenic banana lines were divided into three types and the transformed fragments were high copy insertion. The Mh-ACO2 was decreased in different lines and different organs in transgenic bananas. The siRNAs were only expressed in petal. Mh-ACO1 was increased in wounded leaves but Mh-ACO2 was not. When compared with wild type banana, the change of peel color, ethylene production and respiration rate in MAO2 silenced transgenic banana fruits was delayed. The Mh-ACO1 and Mh-ACO2 gene expression pattern in peel and pulp were different.
Subjects
transgenic banana
ripen delayed
ethylene
acc oxidase
RNA interference
Type
thesis
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