Effects of Industrial Thermal Treatments on the Release of Bovine Colostrum Glycoprotein N-Glycans by Endo-β-N-acetylglucosaminidase
Journal
Journal of Agricultural and Food Chemistry
Journal Volume
68
Journal Issue
51
Start Page
15208
End Page
15215
Date Issued
2020-12-23
Author(s)
Bunyatratchata, Apichaya
Ozturk, Gulustan
Cohen, Joshua L.
Bhattacharya, Mrittika
Mln De Moura Bell, Juliana
Barile, Daniela
Abstract
N-Glycans are structurally similar to human milk oligosaccharides, the gold standard prebiotics for infants. Bovine milk N-glycans released by endo-β-N-acetylglucosaminidase (EndoBI-1) were shown to have similar prebiotic selectivity as human milk oligosaccharides, explaining the interest for N-glycan recovery for use as prebiotics. Industrial thermal treatments such as high-temperature short-time (HTST) and ultra-high-temperature (UHT) might favor the enzymatic deglycosylation of N-glycans through promoting protein denaturation. We investigated the effects of HTST (72 °C for 15 s) and UHT (135 °C for 3 s) on N-glycan release from bovine colostrum glycoproteins by nonimmobilized and amino-immobilized EndoBI-1. A total of 104 N-glycans including isomers/anomers were identified by high-resolution mass spectrometry. In both EndoBI-1 forms, HTST increased the release of N-glycans; however, the impact of UHT on releasing N-glycans was comparable to the nonthermal treatment. Although the amino-immobilized enzyme similarly released neutral N-glycans as the free form, it released fewer sialylated and fucosylated N-glycans.
Subjects
bovine milk
immobilized enzyme
mass spectrometry
N-glycans
prebiotics
Type
journal article