In vivo Tumor Detection Method by Using Multi-functional Contrast Agent for MRI and Optical System
Date Issued
2009
Date
2009
Author(s)
Chen, Ching-Tang
Abstract
Molecular imaging has become an indispensable technology in cancer research and clinical use. The goal of this study is to combine magnetic resonance imaging and optical imaging system with multifunctional contrast agent to detect xenograft non-small cell lung cancer (NSCLC) murine model.igh temperature solution phase reaction led to 8 nm ultrasmall superparamagnetic iron oxide (USPIO, Fe3O4), and the CdS-capped CdTexSe1-x alloyed quantum dot was synthesized to near-IR emitting nanoparticles. Aqueous iron oxide and near-IR quantum dot nanoparticles were conjugated with anti-epideremal growth factor receptor (EGFR) antibody as the biomedical probe to detect the NSCLC tumor. Variation of T2 relaxation time was obtained from MRI for nano-contrast agent quantification.russian blue staining imaging and third harmonic generation (THG) imaging showed different targeting efficiency in A549 and CL1-0 in vitro. T2 and T2* MR imaging showed significant signal decrease (>30%) in vivo. It was proved caused by nano-probe targeting by using both histological cytochemistry staining and THG imaging. ultifunctional nanocontrast agent could hopefully not only serves as cancer detection and treatment but also used to predict disease prognosis in the future.
Subjects
multifunctional
nano-contrast agent
Non-small lung cancer
imaging quantification
SDGs
Type
thesis
File(s)![Thumbnail Image]()
Loading...
Name
ntu-98-R96945037-1.pdf
Size
23.32 KB
Format
Adobe PDF
Checksum
(MD5):1dfd5ab462daf289e0cb192e6cc4f7f1